HBSS buffer (1X Hank’s Balanced Salt Solution with 20mM HEPES buffer, pH 7.3)

Hanks' Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological pH for cells maintained in non-CO2 atmospheric conditions.  This formulation is produced with calcium and magnesium, and without phenol red.

HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) proveds an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2.  HEPES has a pKa of 7.66 making it a much stronger buffer in the pH 7.2-7.6 range than bicarbonate buffer.  The recommended working concentration is 10-25 mM.

components manufactured and assembled for TEFLabs by Mediatech, Inc.