- #3500 ANG-1 (AM) 500 ug... $250*OUT OF STOCK- consider #3502 ANG-2
- #3510 ANG-1 (AM) 10 x 50 ug... $300*OUT OF STOCK- consider #3512 ANG-2
- #3520 ANG-1 (TMA+ salt) 250 ug... $125 Add to Cart
- #3530 ANG-1 (AM) 5 x 500 ug... $1000*OUT OF STOCK- consider #3532 ANG-2
- #3540 ANG-1 (AM) 2 x 50 ug... $65*OUT OF STOCK- consider #3542 ANG-2
- #3550 ANG-1 (TMA+ salt) 2 x 25 ug... $30 Add to Cart
Asante NaTRIUM Green-1 is a visible wavelength fluorescent indicator with a useful dynamic range for measuring cytosolic Na+ concentrations. Unlike SBFI, it loads readily into cells and is excited by visible light. Although ANG-1 excites maximally at 517 nm, its exceptional brightness enables excitation at the standard 488 nm settings used for the Fluo calcium indicators. Moreover, it works well for 2-photon excitation with near-infrared light. ANG-1 is also remarkable in its resistance to photobleaching and leakage.
Looking for a lower kd? Try Asante Natrium Green-2.
DATA
Excitation: 488-517 nm (excitation maximum at 517 nm)
Emission: 540 nm
Kd: 85 mM
Solubility: H2O (salt form) DMSO (AM form)
Molecular Weight: 1100 g/mol
RESULTS
Publication:
A recent article by Christophe Lamy, and Jean-Yves Chatton highlights the uses and improved performance of TEFLabs' novel sodium indicator, Asante NaTRIUM Green-1™.
ANG-1 Emission Spectra
Screening mode (FIG. 2.1): HEK293 cells expressing TRPV1 channels were loaded with ANG-1. Capsaicin, an agonist for TRPV1 channels, which conduct Na+ and Ca2+, was used to stimulate Na+ influx. The capsaicin-evoked rise in ANG-1 fluorescence is robust. [J. Kao, Univ. of Maryland]
FIG. 2.1 ANG-1 in HEK293 cells
Flow cytometry and wide-field microscopy mode (FIG. 2.2): REF52 cells were loaded with ANG-1, and the Na+ ionophore gramicidin was applied to promote Na+ influx . in the resulting rise in intracellular Na+ concentration caused a corresponding increase in ANG-1 fluorescence. [J. Kao, Univ. of Maryland]
FIG. 2.2 ANG-1 in REF52 cells
Confocal microscope mode (FIG. 2.3): Astrocytes were loaded with ANG-1 and ouabain, a sodium pump inhibitor, was used to block Na+ extrusion from the cell. The second frame shows the consequent increase in ANG-1 fluorescence from a baseline after only three minutes of treatment with ouabain. (Courtesy JY Chatton, Univ of Lausanne)
Baseline Ouabain(3 min.)
FIG. 2.3
ANG-1 (AM) cell loading procedure for the experiments of Figures 2.1 and 2.2. (This procedure should not be treated as a general loading protocol for ANG-1. Please refer to Chapter 1 for general loading guidelines.)
(i) 1-5 µM of ANG-1 (AM) is an average concentration.
(ii) The sample is prepared with ~75 ppm Pluronic F-127 for dispersing the dye into the incubator buffer.
(iii) For these experiments, the buffer consisted of HCO3- DMEM with 10% fetal bovine serum (FBS) and cells were kept under 5% CO2 atmosphere.
(iv) Loading time varied from 45 minutes to 70 minutes.
(v) The experiments were conducted in HBSS.
[J. Kao of Univ. of Maryland Medical School]