- #2510 Pluronic F127 20% (wt.v) in DMSO 1 ml...$29 Add to Cart
- #2512 Pluronic F127 20% (wt.v) in DMSO 100 ml...$150 Add to Cart
- #2514 Pluronic F127 7% (wt.v) in DMSO 10 x 50 uL...$20
- #2516 Pluronic F127 7% (wt.v) in DMSO 500 uL...$15
Pluronic F-127 is used to help the acetoxymethyl (AM) esters of fluorescent ion indicators disperse. Pluronic facilitates the solubilization of water-insoluble indicators. For many indicators provided by TEFLabs, pluronic may be required for optimal loading condiions.
Pluronic should only be added to working solutions. A 1-5 mM AM ester solution dissolved in DMSO may be mixed with 20% (wt/v) Pluronic F-127 stock solution in DMSO in a 1:1 ratio immediately prior to use. The AM ester and pluronic solution is then diluted into a cell-loading buffer solution to achieve the final loading concentration between 1μM and 1o μM. Cells are then incubated between 30-60 minutes. Pluronic concentrations are typicall kept at .1%.
Difficult to load dyes such as Asante Calcium Red, SBFI, PBFI will require more concentrated loading solutions and correspondingly, greater pluronic concentrations.
TEFLabs offers Pluronic F127 20% (wt/v) in DMSO in 1 ml solutions.