Pluronic

  • #2510 Pluronic F127 20% (wt.v) in DMSO  1 ml...$29 Add to Cart
  • #2512 Pluronic F127 20% (wt.v) in DMSO  100 ml...$150 Add to Cart
  • #2514 Pluronic F127 7% (wt.v) in DMSO  10 x 50 uL...$20
  • #2516 Pluronic F127 7% (wt.v) in DMSO  500 uL...$15

Pluronic F-127 is used to help the acetoxymethyl (AM) esters of fluorescent ion indicators disperse.  Pluronic facilitates the solubilization of water-insoluble indicators.  For many indicators provided by TEFLabs, pluronic may be required for optimal loading condiions.

Pluronic should only be added to working solutions.  A 1-5 mM AM ester solution dissolved in DMSO may be mixed with 20% (wt/v) Pluronic F-127 stock solution in DMSO in a 1:1 ratio immediately prior to use. The AM ester and pluronic solution is then diluted into a cell-loading buffer solution to achieve the final loading concentration between 1μM and 1o μM.  Cells are then incubated between 30-60 minutes. Pluronic concentrations are typicall kept at .1%.

Difficult to load dyes such as Asante Calcium Red, SBFI, PBFI will require more concentrated loading solutions and correspondingly, greater pluronic concentrations.

TEFLabs offers Pluronic F127 20% (wt/v) in DMSO in 1 ml solutions.