TEFLabs continues to offer our protein kinase C (PKC) indicators developed at the University of Texas by Poenie and coworkers. The general feedback is that Rim-1 is more stable and cell-permeable. Fim-1 in the K+ salt form is cell-impermeant; Fim-1 diacetate is cell-permeant and thus can be used for loading the indicator into cells by incubation. Table 10.2 lists the properties of the PKC indicators.
Table 10.2 PKC Indicators