Fluo-8

Fluo-8 has become the most popular of many names used to market a specific version of the molecule patented and introduced to the world as Fluo-2 in 1988-1989 (US Patent 5049673, 1991). Fluo-8 is Fluo-2 Medium Affinity which replaces the Methyl group of the Fluo-2 High Affinity with a Hydrogen as described below.

Fluo-8 Data

  • Excitation: 488 nm
  • Emission: 515 nm
  • kd: 390 nM
  • Solubility: H20 (salt form) DMSO (AM form)
  • Molecular Weight: 877 g/mol (salt) 1047 g/mol (AM)

Documents

Spec Sheets
Info Pack
Material Safety Data Sheet

Fluo-8 Overview

Fluo-8 has been found to be much brighter (1.5x) than Fluo-4 in cellular experiments. The increased brightness of Fluo-8  is partly attributable to its superior loading in most cell types. TEFLabs Fluo-8 (and thus Fluo-2 Medium Affinity) offer improved cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation at 490 nm and maximum emission at 520 nm. Fluo-8 loadng can be performed at room temperature. 

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Fluo-8 Catalog Numbers

  • #0201 TEFLabs Fluo-8 (AM) 1mg... $110
  • #0203 TEFLabs Fluo-8 (AM) 20 x 50 ug... $130
  • #0205 TEFLabs Fluo-8 (salt) 1 mg... $110
  • #0215 TEFLabs Fluo-8 (AM) 2 x 50 ug... $15
  • #0217 TEFLabs Fluo-8 (salt) 2 x 25 ug... $15

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