Quin-2 (Figure 5.3) was the first fluorescent calcium indicator made by Dr. Tsien after he invented BAPTA as the precursor to all calcium indicators. It is still used today, but the higher quantum yield of Fura-2 has all but replaced it as an excitation ratio indicator. It has an excitation ratio of 383 nm/ 353 nm with emission at 495 nm.
DATA
Excitation: 333/353 nm (high/low Ca2+)
Emission: 495 nm
kd: 60 nM
Solubility: H20 (salt form) DMSO (AM form)
Molecular Weight: 542 g/mol (salt form), 830 g/mol (AM form)
RESULTS
Table 5.1 UV-excitable Calcium Indicators
Fig 5.3 Quin-2 Structure
RELATED PRODUCTS
Asante Calcium Red (ACR)
Asante Calcium Green (ACG)
Fluo-2