Unlike other hydrophobic dyes, TEFLabs near membrane dyes have a piperazine collar to latch the dye into the membrane, leaving the chelating portion to measure calcium in the gates. Our near-membrane UV-excitable indicators Fura-2 NearMem (formerly FFP-18) , and Indo-1 NearMem (formerly FIP-18), have enjoyed considerable success for their applications, because unlike other hydrophobic indicators, they do not get lost in the membrane.
Indo-1 is also available in High Affinity (Indo-1), Low Affinity (Indo-1 LowAff), and Leakage Resistant (Indo-1 LeakRes) versions.
DATA
Excitation: 346 nm
Emission: ratiometric, 475 nm and 405 nm
kd: 450 nM
Solubility: H20 (salt form) DMSO (AM form)
Molecular Weight: 1134 g/mol (salt), 840 g/mol (AM)
RESULTS
FIG. 6.10 Indo-1 NearMem (FIP-18)
RELATED PRODUCTS
Indo-1
Indo-1 LeakRes
Indo-1 LowAff
Asante Calcium Red Near Membrane
Fluo-2 Near Membrane
Fura-2 Near Membrane