Indo-1 Low Affinity (LowAff)
- #0138 Indo-1 LowAff (AM) 1mg... $110 Add to Cart
- #0139 Indo-1 LowAff (AM) 20 x 50 ug... $130 Add to Cart
- #0140 Indo-1 LowAff (salt) 1 mg... $110 Add to Cart
Low affinity (formerly designated as “FF”) versions enable studies where a large concentration of calcium is expected. They have two major advantages over their high affinity counterparts. They cause reduced buffering of intracellular calcium and reduced perturbation of calcium transients, and they allow for measurements of shorter-lived transients.
TEFLabs low affinity calcium indicators, based on the effect of fluorines on lowering the affinity of the regular indicators, have an appropriate Kd in the range of 15 μM to 25 μM. Fura-2 LowAff and Indo-1 LowAff were the first low affinity indicators introduced by TEFLabs.
Excitation: 346 nm (high/low Ca2+)
Emission: ratiometric, 475 nm and 405 nm
kd: 26 uM
Solubility: H20 (salt form) DMSO (AM form)
Molecular Weight: 1046 g/mol (AM form), 862 g/mol (salt form)
Figure 6.2 Indo-1 LowAff Structure