Unlike other hydrophobic dyes, TEFLabs near membrane dyes have a piperazine collar to latch the dye into the membrane, leaving the chelating portion to measure calcium in the gates. Our near-membrane UV-excitable indicators Fura-2 NearMem (formerly FFP-18) , and Indo-1 NearMem (formerly FIP-18), have enjoyed considerable success for their applications, because unlike other hydrophobic indicators, they do not get lost in the membrane.
Fura-2 is also available in High Affinity (Fura-2), Low Affinity (Fura-2 LowAff), and Leakage Resistant (Fura-2 LeakRes) versions.
DATA
Excitation: 340/380 nm (high/low Ca2+)
Emission: 505 nm
kd: 400nM
Solubility: H20 (salt form) DMSO (AM form)
Molecular Weight: 1164 g/mol(salt form), 1296 g/mol(AM form)
RESULTS
FIG. 6.9 Fura-2 NearMem Structure
FIG 6.11
FIG.6.12
Figure 6.11 shows the distribution of the dye Fura-2 NearMem in the plasma membrane. Figure 6.12 is a plot of fluoresence intensity due to Fura-2 NearMem as a function of the distance from the cell membrane. FIG. 6.12 is a plot of the excitation spectra of a titration of Fura-2 NearMem with calcium, where emission was set at 500 nm.
RELATED PRODUCTS
Fura-2
Fura-2 LeakRes
Fura-2 LowAff
Asante Calcium Red Near Membrane
Fluo-2 Near Membrane
Indo-1 Near Membrane