Fura-2 Near Membrane (NearMem)

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Unlike other hydrophobic dyes, TEFLabs near membrane dyes have a piperazine collar to latch the dye into the membrane, leaving the chelating portion to measure calcium in the gates. Our near-membrane UV-excitable indicators Fura-2 NearMem (formerly FFP-18) , and Indo-1 NearMem  (formerly FIP-18), have enjoyed considerable success for their applications, because unlike other hydrophobic indicators, they do not get lost in the membrane.

Fura-2 is also available in High Affinity (Fura-2), Low Affinity (Fura-2 LowAff), and Leakage Resistant (Fura-2 LeakRes) versions.


Excitation: 340/380 nm (high/low Ca2+)

Emission:  505 nm

kd:  400nM

Solubility: H20 (salt form)  DMSO (AM form)

Molecular Weight:  1164 g/mol(salt form), 1296 g/mol(AM form)


Fura-2 NearMem, FFP-18 structure

FIG. 6.9 Fura-2 NearMem Structure


describe the image

FIG 6.11


Fura-2 NearMemFIG.6.12

Figure 6.11 shows the distribution of the dye Fura-2 NearMem in the plasma membrane.  Figure 6.12 is a plot of fluoresence intensity due to Fura-2 NearMem as a function of the distance from the cell membrane.   FIG. 6.12 is a plot of the excitation spectra of a titration of Fura-2 NearMem with calcium, where emission was set at 500 nm.



Fura-2 LeakRes

Fura-2 LowAff

Asante Calcium Red Near Membrane

Fluo-2 Near Membrane

Indo-1 Near Membrane