Fura-2 Near Membrane (NearMem)
- #0125 Fura-2 NearMem (AM) 1mg... $110 Add to Cart
- #0126 Fura-2 NearMem (AM) 20 x 50 ug... $130 Add to Cart
- #0111 Fura-2 NearMem (salt) 1 mg... $110 Add to Cart
Unlike other hydrophobic dyes, TEFLabs near membrane dyes have a piperazine collar to latch the dye into the membrane, leaving the chelating portion to measure calcium in the gates. Our near-membrane UV-excitable indicators Fura-2 NearMem (formerly FFP-18) , and Indo-1 NearMem (formerly FIP-18), have enjoyed considerable success for their applications, because unlike other hydrophobic indicators, they do not get lost in the membrane.
Excitation: 340/380 nm (high/low Ca2+)
Emission: 505 nm
Solubility: H20 (salt form) DMSO (AM form)Molecular Weight: 1164 g/mol(salt form), 1296 g/mol(AM form)
FIG. 6.9 Fura-2 NearMem Structure
Figure 6.11 shows the distribution of the dye Fura-2 NearMem in the plasma membrane. Figure 6.12 is a plot of fluoresence intensity due to Fura-2 NearMem as a function of the distance from the cell membrane. FIG. 6.12 is a plot of the excitation spectra of a titration of Fura-2 NearMem with calcium, where emission was set at 500 nm.