Fura-2 Leakage Resistant (Fura-PE3)

  • #0108 Fura-2 LeakRes (AM) 1mg... $110 Add to Cart
  • #0109 Fura-2 LeakRes (AM) 20 x 50 ug... $130 Add to Cart
  • #0110 Fura-2 LeakRes (salt) 1 mg... $110 Add to Cart
  • #0116 Fura-2 LeakRes (AM) Trial Size 2 x 50 µg... $20 Add to Cart
  • #0118 Fura-2 LeakRes (salt) Trial Size 2 x 50 µg... $20 Add to Cart

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Leakage resistance (LeakRes, formerly PE3) versions have a special appendage that increases the intracellular retention – well in excess of an hour.

Compared to the corresponding non-LeakRes versions, the LeakRes indicators are identical in fluorescence prperties but are retained in the cell for hours.  In addition to Fura and Indo, TEFLabs has applied this unique technology to our new indicators Asante Calcium Red and Fluo-2. 

Fura-2 is also available in High Affinity (Fura-2), Low Affinity (Fura-2 LowAff), and Near Membrane (Fura-2 NearMem) versions.

DATA

Excitation: 340/380 nm (high/low Ca2+)

Emission:  505 nm

kd: 145 nM (224 at 37)

Solubility: H20 (salt form)  DMSO (AM form)

Molecular Weight:  832 g/mol

RESULTS

 

Figure 6.7 Retention of Fura-2 LeakRes and leakage of Fura-2:  322 T lymphoma cells wer loaded with either Fura-2 or Fura-2 LeakRes and set in calcium buffer.  Leakage of Fura-2 or Fura-2 LeakRes into the exterior calcium buffer resulted in increased fluorescence overall.  This fluorescence was plotted over time.

Figure 6.7 Retention of Fura-2 LeakRes and leakage of Fura-2:  322 T lymphoma cells wer loaded with either Fura-2 or Fura-2 LeakRes and set in calcium buffer.  Leakage of Fura-2 or Fura-2 LeakRes into the exterior calcium buffer resulted in increased fluorescence overall.  This fluorescence was plotted over time.

 

 

fura-2 vs furaPE3
Figure 6.8 shows the slower decrease in fluorescence due to leakage of indicator.  The top row shows images of BPV cells loaded with Fura-2 LeakRes, whereas the bottom row shows BPV cells loaded with Fura-2.  Images were taken at every 20 minutes.  Fura-2 cells show loss of significant fluorescence by T = 40 minutes.  Fura-2 LeakRes cells retain fluorescence even at T = 100 minutes.

RELATED PRODUCTS

Fura-2

Fura-2 NearMem

Fura-2 LowAff

Asante Calcium Red Leak Res

Asante Calcium Green (ACG)

Fluo-2 Leak Res

Indo-1 Leak Res

Pluronic