- #0200 Fluo-2 MedAff (AM) 1mg... $110 Add to Cart
- #0202 Fluo-2 MedAff (AM) 20 x 50 μg... $130 Add to Cart
- #0204 Fluo-2 MedAff (salt) 1 mg... $110 Add to Cart
- #0211Fluo-2 MedAff (AM) 10 x 30 μg... $40
- #0214 Fluo-2 MedAff (AM) 2 x 50 μg... $15 Add to Cart
- #0216 Fluo-2 MedAff (salt) 2 x 25 μg... $15 Add to Cart
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Fluo-2 is one of the original Fluo series of Ca2+ indicators invented by Drs. Roger Tsien and Akwasi Minta in the 1980s. Whereas Fluo-3 and Fluo-4 were commercialized, Fluo-2 was not. Subsequently, Fluo-2 Medium Affinity (Fluo-2 MedAff) has been found to be much brighter (1.5x) than Fluo-4 in cellular experiments, as shown in Figures 4.11 and 4.12. The increased brightness of Fluo-2 MedAff is partly attributable to its superior loading in most cell types. Although a lower pKa was assumed necessary when the Fluo dyes were invented, experimental data has established that Fluo-2’s pKa is not a limitation.
For improved performance over Fluo indicators, TEFLabs recommends Asante Calcium Green (substantially enhanced brightness and dynamic range) and, for some applications, Asante Calcium Red (fluorescence emission at much longer wavelength).
DATA
Excitation: 488 nm
Emission: 515 nm
kd: 390 nM
Solubility: H20 (salt form) DMSO (AM form)
Molecular Weight: 877 g/mol (salt) 1047 g/mol (AM)
RESULTS
The protocol for using Fluo-2 is the same that is used for Fluo-4.Typical loading protocol for Fluo-2
- Prepare stock solution of 1 – 10 mM Fluo-2 (AM) in DMSO
- Dilute AM stock solution to 1 – 5 μM in aqueous medium and incubate cells for 30 – 60 minutes at room temperature. (If necessary, premix Pluronic F-127 stock solution (15% wt/vol in DMSO) with the desired volume of Fluo-2 (AM) stock solution before dispersal into aqueous medium.)
- Wash/transfer cells into fresh medium for experimentation (for “wash-free” assays, washing may be omitted).
Figure. 4.10 Fluo-2, 3, 4 structures
Table 4.6 Fluo-2 versus Fluo-4 comparison
Table 4.6 shows a comparison of Fluo-2 MedAff (Fluo-2 Medium Affinity) and Fluo-4 properties. the pKa of Fluo-2 MedAff is slightly higher than Fluo-4. Although a lower pKa was preferred when the Fluo dyes were invented, experimental data has established that Fluo-2’s pKa is not a limitation. (See History of Fluo Dyes)
Figure. 4.11 FLUO-2,3, 4 brightness comparison
Figure. 4.12 FLUO-2, 4 cell comparison
Table 4.7 FLUO-2 properties
RELATED PRODUCTS
TEFLabs Fluo-8
Fluo-2 HighAff
Fluo-2 LowAff
Fluo-2 LeakRes
Fluo-2 NearMem
Asante Calcium Red (ACR)
Asante Calcium Green