Asante Calcium Red

Asante Calcium Red emits fluorescence at long wavelengths, where there is virtually no interference from cellular autofluorescence. Exciting at 488 nm produces dual fluorescence emission at 650 and 525 nm, enabling emission ratiometry. ACR can also be excited at 540 nm to generate fluorescence emission at 650 nm, which can be used for non-ratiometric measurement.

ACR is also available in a Low Affinity (Asante Calcium Red LowAff) version and Near Membrane(Asante Calcium Red NearMem) and Leakage Resistant (Asante Calcium Red LeakRes) versions are currently under development.

ACR in Ratiometric Mode

  • Excitation: 488 nm
  • Emission: 525/650 nm
  • kd: 400 nM
  • Solubility: H20 (salt form) DMSO (AM form)
  • Molecular Weight: 1000g/mol (salt) 1200 g/mol (AM)

ACR in Non-Ratiometric Mode

  • Excitation: 540 nm
  • Emission: 650 nm
  • kd: 400 nM
  • Solubility: H20 (salt form) DMSO (AM form)
  • Molecular Weight: 1000g/mol (salt) 1200 g/mol (AM)

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Spec Sheets
Info Pack

ACR in Ratiometric Mode

Ratiometry offers ease of calibration and enables quantitative measurement by eliminating variables such as indicator concentration, photobleaching, indicator leakage, and cell thickness. Importantly, a red-emitting indicator significantly reduces interference from cellular autofluorescence, which typically occurs at shorter wavelengths (e.g., flavin nucleotide cofactors, FMN and FAD, emit green fluorescence peaking at ~540 nm).

ACR in Non-Ratiometric Mode

When excited at 540 nm (Figure. 4.1), ACR emits maximally at 650 nm; it shows a 50-fold increase in intensity on going from the Ca2+-free to the Ca2+-bound form. The red fluorescence of ACR is not as intense as the green fluorescence of dyes, but is useful for many applications.

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ACR Catalog Numbers

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