Asante Calcium Green

ACG’s high fluorescence quantum efficiency makes it the brightest Ca2+ indicator with the highest fluorescent dynamic range to date. This exceptional brightness and dynamic range allows ACG to report both small and large Ca2+ signals with good signal-to-noise.TEFLabs plans to offer multiple versions of ACG with a range of Kds. Improved methods of non-invasive cell loading are being developed

Asante Calcium Green Data

  • Excitation: 517 nm
  • Emission: 540 nm
  • kd: 135 nM
  • Solubility: H20 (salt form) DMSO (AM form)
  • Molecular Weight: 1200g/mol (salt) 1400 g/mol (AM)

Documents

Spec Sheets
Info Pack
Material Safety Data Sheet

Asante Calcium Green Overview

In the past, the nonratiometric Fluo dyes have been very useful and popular because they have a large dynamic range (Fmax/Fmin ≈ 100). Asante Calcium Green (ACG) is much brighter than the Fluo dyes with an even larger dynamic range: Fmax/Fmin = 220 (Figure 4.7), where the relevant quantum efficiencies are: Ca2+-free Q = 0.00225, Ca2+-bound Q = 0.495. Figure 4.8 compares the quantum efficiency of Ca2+-bound ACG to fluorescein.

Read More

Asante Calcium Green Catalog Numbers

  • #3700 ACG (AM) 500 ug...UNAVAILABLE
  • #3702 ACG (AM) 10 x 50 ug...UNAVAILABLE
  • #3704 ACG (K+salt) 250 ug...$125
  • #3706 ACG (AM) 5 x 500 ug...UNAVAILABLE
  • #3708 ACG (AM) 2 x 50 ug...UNAVAILABLE
  • #3710 ACG (K+salt) 2 x 25 ug...$30

All Content Copyright © 2011 by TEFLabs