Asante Natrium Green in Nav 1.3
Posted on Tue, Oct 18, 2011
A preliminary response of Asante Natrium Green-2 with sodium channel receptor, Nav 1.3 expressed in HEK 293 cells is shown in Figure 1 below. The wavelength of excitation used was 488 nm. Figure 2 shows various excitation wavelengths including emission maxima 517 nm. Filters or Flexstation may be neccessary for maximum response.
Figure 1: Na+ flux in NaV 1.3-expressing HEK-293 cells loaded with ANG-2 and treated with a concentration series of lidocaine. Cells stimulated with 25 mM vertradine in 20 mM HEPES-buffered HBSS.
Figure 2 (below): The maximum emission reached on ANG-2TMA+ at different excitation Wavelengths.
