Fluo-2 vs Fluo-3, Fluo-4, and Fluo-8

  

 Email Article  

Tweet  

  

FLUO-2 MA (AM), US Patent # 5,049,673
(FLUO-2 Medium Affinity, Acetoxymethyl ester)

Fluo-2 Medium Affinity emerges as an ideal candidate over Fluo-3 and Fluo-4 for calcium study.  With improved loading characteristics and brightness (nearly 2x as bright as Fluo-4, 4 x as bright as Fluo-3) Fluo-2 MA (the same molecule as Fluo-8) has become a very cost effective replacement or screeners and researchers currently using Fluo-3, Fluo-4 or Fluo-8

Fluo-2 MA, Fluo-3, Fluo-4, Fluo-8 Comparison
REF52 rat embryo fibroblasts

Procedure:

REF52 rat embryo fibroblasts, cultured on No. glass coverslips, were incubated for 45 minutes at room temperature with 5 μM of the AM ester of each indicator in serum-free DMEM containing 25 mM HEPES (pH 7.4). The coverslips were then rinsed in HBSS, mounted in round dishes and bathed with HBSS. Indicators were excited with 488-nm light from a monochromator; fluorescence images were acquired with a cooled CCD camera through a ×40 objective with high numerical aperture (NA = 1.4). Cells were stimulated with 2 μM ionomycin to elicit a sharp rise in intracellular Ca2+ concentration. The images acquired immediately before and after ionomycin addition are shown. The fluorescence change resulting
from ionomycin application in each population of cells was also analyzed; the results are displayed in the bar graph. The result for each indicator is derived from analyzing 35 – 45 cells.

Fluo-2 Products currently available:

Fluo-2 MA (Medium Affinity): Excitation at 490 nm, emission at 515 nm, and 390 nM Kd

TRIAL SIZE #0214 Fluo-2 MA (AM) 2 x 50 ug  $15

TRIAL SIZE #0216 Fluo-2 MA (K+SALT) 2 x 50 ug  $15

#0200 Fluo-2 MA (AM) 1 mg  $110

#0202 Fluo-2 MA (AM) 20 x 50 ug  $130

#0204 Fluo-2 MA (K+SALT) 1 mg  $110

Fluo-2 HA (High Affinity): Excitation at 490 nm, emission at 515 nm

#0220 Fluo-2 HA (AM) 1mg  $110

#0222 Fluo-2 HA (AM) 2 x 50 ug  $130

#0224 Fluo-2 HA (K+SALT 1mg  $110

Fluo-2 LA (Low Affinity):  Excitation at 490 nm, emission at 515 nm

#0240 Fluo-2 LA (AM) 1mg  $130

#0242 Fluo-2 LA (AM) 2 x 50 ug  $150

#0244 Fluo-2 LA (K+SALT) 1mg  $130

Fluo-2 LeakRes (Leakage  Resistant):  Excitation at 490 nm, emission at 515 nm

#0230 Fluo-2 LeakRes (AM) 1mg  $130

#0232 Fluo-2 LeakRes (AM) 2 x 50 ug  $150

#0234 Fluo-2 LeakRes (K+SALT) 1mg  $130

Bulk discounts available, email probes@teflabs.com for bulk quotes.