Asante NaTRIUM Green Test Results

Test Results and images for TEFLabs' new visible wavelength fluorescent sodium indicator, Asante NaTRIUM Green:

Baseline vs Ouabain after 3min ; Rem: excitation 495nm, emission 515LP,  JY Chatton, University of Lausanne, June 2010

 

REF52 cells loaded 1-2 hr at room temperature with 5 mM ANG AM + 75 ppm Pluronic F-127 in HCO3--buffered DMEM containing 10% fetal bovine serum(FBS), under 5% CO2 atmosphere. Experiments were conducted in HBSS. Excitation l = 488 nm. Each trace is the average response of 26-39 cells. Cells were used immediately after loading. JPY Kao, University of Maryland,  June 2010.

 

 

 

HEK293 cells permanently overexpressing TRPV1, loaded 70-80 min at room temp. with 5 mM ANG AM + 75 ppm Pluronic F-127 in HCO3--buffered DMEM containing 10% fetal bovine serum (FBS), under 5% CO2 atmosphere. Experiments were conducted in HBSS. Excitation l = 488 nm. Average response of  108 cells. JPY Kao, University of Maryland,  June 2010.