Product Specification and
Analysis
|
|
|
|
PRODUCT NAME: |
FLUO-4 (AM) [Fluoride
analog of Fluo-3, according to US patent 5,049,673] |
|
|
C51H50F2N2O23 |
|
|
MW = 1096.95 M.W. 1096.95 g/mol |
|
|
Kd =
345 nM (After hydrolysis)* |
|
|
Store Frozen
(-20°C) |
|
|
PROTECT FROM
LIGHT |
|
|
Soluble in DMSO |
|
|
|
|
CAS Number/Name: |
Glycine,
N-[4-[6-[(acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxyethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxyethyl]-,
(acetyloxy)methyl ester |
|
DESCRIPTION: |
Red solid. Cell-permeable acetoxymethyl ester (AM)
derivative of Fluo-4 with spectral properties similar to the parent compound.
Insensitive to magnesium. |
|
CATALOG
NUMBER: |
0151, 0152, 0153 |
|
APPEARANCE: |
Red Glossy Solid |
|
TLC : |
Ethyl Acetate: Hexane 5:2 Rf = 0.2 Purity: 100% |
|
ABSORPTION
SPECTRUM: [of the Acetoxy Methyl (AM) Ester] |
l max: 452 nm (in Ethyl Acetate) Extinction: 25,000 M-1cm-1 |
|
EMISSION
SPECTRUM: |
lmax: 516 nm (after hydrolysis)* |
|
EXCITATION: 1H NMR: |
494 nm (after hydrolysis)* All relevant peaks present (see attached) |
|
HPLC: Solvent
System: Column: |
Purity: 100% (see
attached) Water/MeOH gradient 40-100% C18 |
* Fluorescence
and binding to calcium is only observed after hydrolysis to the Fluo-4 anion.