Product Specification and
Analysis
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PRODUCT NAME: |
INDO-PE3 (AM) |
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C58H67N5O27 |
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M.W. 1266 g/mol |
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Apparent Kd
= 260 nM (pH 7.2) |
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Store Frozen
(-20°C) |
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PROTECT FROM
LIGHT |
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Soluble in DMSO |
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DESCRIPTION: |
Yellow gum. Cell permeable acetoxymethyl ester (AM)
derivative of Indo-PE3 with spectral properties similar to the parent
compound. A ratiometric dye with the same fluorescent properties and similar
applications as Indo-1, but is leakage resistant. |
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CATALOG
NUMBER: |
0144 & 0145 |
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APPEARANCE: |
Yellow gum |
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TLC : |
Ethyl Acetate:Hexane Purity: 100% |
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ABSORPTION
SPECTRUM: |
l max (low
Ca2+): 346 nm Extinction: 33,000 M-1cm-1 |
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EMISSION
SPECTRUM: |
lmax(Ca2+
free): 475 nm lmax(Ca2+
bound): 408 nm |
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EXCITATION: |
345-360 nm |
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HPLC: Solvent
System: Column: |
Purity: ~90% Water/MeOH gradient 40-100% C18 |
