Product Specification and
Analysis
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PRODUCT NAME: |
FLUO-3FF(Pentapotassium
Salt) |
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C35H21N2O13F2Cl2K5 |
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M.W. 982 g/mol |
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Kd =
42 mM (35°C) |
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Store Frozen
(-20°C) |
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PROTECT FROM
LIGHT |
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Soluble in H2O |
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DESCRIPTION: |
Red solid. A non-ratiometric dye with the same fluorescent
properties and similar applications as FLUO-3, but has a lower affinity for
calcium, and is insensitive to magnesium. |
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CATALOG
NUMBER: |
0143 |
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APPEARANCE: |
Red Solid |
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TLC : |
Reverse Phase (H2O/MeOH) Rf = Solvent front Purity: 100% |
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ABSORPTION
SPECTRUM: |
l max: 515 nm Extinction: 80,000 M-1cm-1 |
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EMISSION
SPECTRUM: |
lmax: 526 nm |
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EXCITATION: |
490-510 nm |
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HPLC: Solvent
System: Column: |
Purity: ~90% Water/MeOH gradient 40-100% C18 |
Ref.: Zoltan
Cseresnyes, Alexander I. Bustamante, Michael G. Klein, and Martin F. Schneider.
(1997) Release-Activated Ca 2+
Transport in Neurons of Frog Sympathetic Ganglia. Neuron 19: 403-419.
David, G. ,
Barrett, J. N., Barrett, E. F. (1997) Stimulation-induced changes in [Ca2+]
in lizard motor nerve terminals. J
Physiol (Lond) 504: 83-96