Product Specification and
Analysis
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PRODUCT NAME: |
FLUO-3FF (AM) |
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C49H46N2O23F2Cl2 |
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M.W. 1139.8 g/mol |
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Kd =
42 mM (35°C) |
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Store Frozen
(-20°C) |
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PROTECT FROM
LIGHT |
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Soluble in DMSO |
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DESCRIPTION: |
Red solid. Cell-permeable acetoxymethyl ester (AM)
derivative of Fluo-3FF with spectral properties similar to the parent
compound. The same fluorescent properties and similar applications as FLUO-3,
but has a lower affinity for calcium, and is insensitive to magnesium. |
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CATALOG
NUMBER: |
0141 & 0142 |
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APPEARANCE: |
Red Solid |
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TLC : |
Ethyl Acetate: Hexane Rf = 0.3 Purity: 100% |
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ABSORPTION
SPECTRUM: |
l max: 462 nm Extinction: 25,000 M-1cm-1 |
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EMISSION
SPECTRUM: |
lmax: 526 nm (after hydrolysis) |
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EXCITATION: |
490-510 nm |
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HPLC: Solvent
System: Column: |
Purity: >95% Water/MeOH gradient 40-100% C18 |
Ref.: Zoltan
Cseresnyes, Alexander I. Bustamante, Michael G. Klein, and Martin F. Schneider.
(1997) Release-Activated Ca 2+
Transport in Neurons of Frog Sympathetic Ganglia. Neuron 19:403-419.
David, G.,
Barrett, J.N., Barrett, E. F. (1997) Stimulation-induced changes in [Ca2+]
in lizard motor nerve terminals. J
Physiol (Lond) 504:83-96.
NOTE:
Loading time may be as long as 1.5-2 hours