Product Specification and
Analysis
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PRODUCT NAME: |
INDO-1FF(AM) |
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C46H47N3O22F2 |
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M.W. 1031.9 g/mol |
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Kd =
26 mM (pH 7.4, 35°C) |
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Store Frozen
(-20°C) |
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PROTECT FROM
LIGHT |
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Soluble in DMSO |
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DESCRIPTION: |
Pale yellow green solid. Cell permeable acetoxymethyl
ester (AM) derivative of Indo-1FF with spectral properties similar to the
parent compound. A ratiometric dye with the same fluorescent properties and
similar applications as Indo-1, but has a lower affinity for calcium, and is
insensitive to magnesium. |
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CATALOG
NUMBER: |
0139 |
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APPEARANCE: |
Pale Yellow Green Solid |
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TLC : |
3:2 Ethyl Acetate:Hexane Rf = 0.6 Purity: 100% |
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ABSORPTION
SPECTRUM: |
l max: 348 nm Extinction: 33,000 M-1cm-1 |
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EMISSION
SPECTRUM: |
lmax: 485 nm (Ca2+ free), ~400 nm (Ca2+
bound) |
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EXCITATION: |
345-360 nm |
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HPLC: Solvent
System: Column: |
Purity: ~90% Water/MeOH gradient 40-100% C18 |
Ref.: Nuesse,
O., Serrander, L., Lew, D.P., Krause, K.-H.(1998) Ca2+-induced
exocytosis in individual human neutrophils: high and low affinity granule
populations and submaximal responses. The
EMBO Journal 17:1279-1288.
Ganitkevich, V.Y.
(1998) Use of Indo-1FF for measurements of rapid micromolar cytoplasmic free Ca2+
increments in a single smooth muscle cell. Cell
Calcium 28:313-322.