Product Specification and
Analysis
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PRODUCT NAME: |
FURA-2FF(Pentapotassium
Salt) |
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LOT |
804 |
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C28H18N3O14F2K5 |
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M.W. 856.0 g/mol |
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Kd
= 6-8 mM (37°C) |
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Store Frozen
(-20°C) |
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PROTECT FROM
LIGHT |
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Soluble in H2O |
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DESCRIPTION: |
Pale yellow solid. A ratiometric dye with the same
fluorescent properties and similar applications as FURA-2, but has a lower
affinity for calcium, and is insensitive to magnesium. |
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CATALOG
NUMBER: |
0137 |
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APPEARANCE: |
Pale Yellow Solid |
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TLC : |
Reverse Phase (H2O/MeOH) Rf = Solvent Front Purity: 100% |
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ABSORPTION
SPECTRUM: |
l max: 360 nm
Extinction: 33,000 M-1cm-1 |
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EMISSION
SPECTRUM: |
lmax: 505 nm |
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EXCITATION: |
380 nm (low Ca2+), 340 nm (high Ca2+) |
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HPLC: Solvent
System: Column: |
Purity: ~90% Water/MeOH gradient 40-100% C18 |
Ref.: Golvina,
V.A., and M.P. Blaustein (1997) Spatially and functionally distinct Ca2+
stores in sarcoplasmic and endoplasmic reticulum. Science 275: 1643-1648.
Joel M. Weinberg,
Julie A. Davis, and Manjeri A. Venkatachalam (1997) Cytosolic-free Calcium
Increases to Greater Than 100 Micromolar in ATP-depleted Proximal Tubules. J.
Clin. Investigations 100:713-722.
Zheng Dong,
Pothana Salkumar, Gary A. Griess, Joel M. Weinberg, and Manjeri A.
Venkatachalam. (1998) Intracellular Ca2+
Thresholds That Determine Survival or Death of Energy-Deprived Cells. Amer. J. Pathology 152:231-240.
Hajnoczy, G. and
Thomas, A. (1997) Minimal Requirements for Calcium Oscillations Driven by the
IP3 Receptor. EMBO Journal 16:3533-3545.