Product Specification and
Analysis
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PRODUCT NAME: |
FFP18 (AM) |
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C62H82N5O25 |
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M.W. 1296 g/mol |
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Apparent Kd
= 400 nM (pH 7.2) |
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Store Frozen
(-20°C) |
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PROTECT FROM
LIGHT |
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Soluble in DMSO
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DESCRIPTION: |
Pale yellow gum. Cell permeable acetoxymethyl ester (AM)
derivative of FFP18 with spectral properties similar to the parent compound.
Selectively monitors near membrane Ca2+ and has low Ca2+
affinity, high solubility in water. |
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CATALOG
NUMBER: |
0125 and 0126 |
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APPEARANCE: |
Pale Yellow Gum |
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TLC : |
Chloroform:methanol
95:5 Rf = 0.6 Purity: 100% |
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ABSORPTION
SPECTRUM: |
l max (low
calcium): 364 nm Extinction:
30,000 M-1cm-1 |
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Emission
Spectrum: |
low calcium: 502 nm |
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HPLC: Solvent
System: Column: |
Purity: >95% Water/MeOH gradient 40-100% C18 |
Ref.:
Davies EV,
Hallett MB Near membrane Ca2+ changes resulting from store release in
neutrophils: detection by FFP-18. Cell Calcium 1996 Apr 19:4 355-62.
Etter EF, Minta
A, Poenie M, Fay FS. Near-membrane [Ca2+] transients resolved using the Ca2+
indicator FFP18. Proc Natl Acad Sci USA 1996 May 93 5368-5373.