Product Specification and
Analysis
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PRODUCT NAME: |
FLUO-3 (AM) |
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LOT: |
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C51H50Cl2N2O23 |
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M.W. 1129.86 g/mol |
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Kd =
390 nM (After hydrolysis)* |
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Store Frozen
(-20°C) |
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PROTECT FROM
LIGHT |
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Soluble in DMSO |
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CAS Number/Name: |
121714-22-5
Glycine,
N-[4-[6-[(acetyloxy)methoxy]-2,7-dichloro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxyethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxyethyl]-,
(acetyloxy)methyl ester |
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DESCRIPTION: |
Red solid. Cell-permeable acetoxymethyl ester (AM)
derivative of Fluo-3 with spectral properties similar to the parent compound.
Insensitive to magnesium. |
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CATALOG
NUMBER: |
0116 AND 0117 |
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APPEARANCE: |
Orange Red Glossy Solid |
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TLC : |
Ethyl Acetate: Hexane 2:1 Rf = 0.4 Purity: 100% |
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ABSORPTION
SPECTRUM: |
l max: 459 nm (Ethyl Acetate) Extinction: 25,000 M-1cm-1 |
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EMISSION
SPECTRUM: |
lmax: 526 nm (after hydrolysis)* |
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EXCITATION: |
490-510 nm (after hydrolysis)* |
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HPLC: Solvent
System: Column: |
Purity: >95% Water/MeOH gradient 40-100% C18 |
* Fluorescence and
binding to calcium is only observed after hydrolysis to the Fluo-3 cation.