Product Specification and Analysis

 

 

 

PRODUCT NAME:

FURA-PE3(Hexapotassium salt)

 

 

 

C38H38N5O17­K6

 

M.W. 1054.3 g/mol

 

Kd = 264 nM (pH 6.9)

 

Store Frozen (-20°C)

 

PROTECT FROM LIGHT

 

Soluble in H2O

 

DESCRIPTION:

 

Off white solid. Fluorescent calcium probe with similar applications and fluorescent properties as Fura-2. Fura-PE3 is retained intracellularly longer than Fura-2.

 

CATALOG NUMBER:

 

0110

 

APPEARANCE:

 

Off white solid

 

TLC :

 

Reverse phase (C18)

Rf =  > 0.9

Purity: 100%

 

ABSORPTION SPECTRUM:

 

l max:   364 nm  Extinction: 33,000 M-1cm-1

 

EMISSION SPECTRUM:

 

lmax (no Ca2+):  502 nm

lmax (high Ca2+):  495 nm

 

EXCITATION:

 

  (no Ca2+): 364 nm

  (high Ca2+): 335 nm

 

HPLC:

Solvent System:

Column:

 

Purity: > 90%

Water/MeOH gradient 40-100%

C18

 

Fura-PE3 References

   1. Ristocetin-mediated interaction of human von Willebrand factor with platelet

     glycoprotein lb evokes a transient calcium signal: observations with Fura-PE3.

     Milner EP, Zheng Q, Kermode JC

     J Lab Clin Med 1998 Jan 131:1 49-62.

   2. Calcium compartments in vascular smooth muscle cells as detected by aequorin

     signal.

     Abe F, Mitsui M, Karaki H, Endoh M

     Br J Pharmacol 1995 Dec 116:7 3000-4.

   3. [Effect of discodermin A, an antimicrobial peptide, on the cytoplasm membrane]

     Sato K, Horibe K, Saito-Mitsui M, Hori M, Matsunaga S, Fusetani N, Ozaki H, Karaki H

     Nippon Yakurigaku Zasshi 1997 Oct 110 Suppl 1: 199P-204P.

   4. New fluorescent calcium indicators designed for cytosolic retention or

     measuring calcium near membranes.

     Vorndran C, Minta A, Poenie M

     Biophys J 1995 Nov 69:5 2112-24.

   5. Palytoxin-induced increase in endothelial Ca2+ concentration in the rabbit

     aortic valve.

     Amano K, Sato K, Hori M, Ozaki H, Karaki H

     Naunyn Schmiedebergs Arch Pharmacol 1997 Jun 355:6 751-8.

   6. Mechanism of contraction induced by bradykinin in the rabbit saphenous vein.

     Eguchi D, Nishimura J, Kobayashi S, Komori K, Sugimachi K, Kanaide H

     Br J Pharmacol 1997 Feb 120:3 371-8.

   7. Calcium-independent regulation of pigment granule aggregation and dispersion

     in teleost retinal pigment epithelial cells.

     King-Smith C, Chen P, Garcia D, Rey H, Burnside B

     J Cell Sci 1996 Jan 109 ( Pt 1): 33-43.

   8. Intracellular calcium release is more efficient than calcium influx in stimulating

     mitochondrial NAD(P)H formation in adrenal glomerulosa cells.

     Rohács T, Tory K, Dobos A, Spaet, A

     Biochem J 1997 Dec 1 328 ( Pt 2): 525-8.

   9. Participation of PKC in modulation of the excitation-contraction process of

     chick embryo cardiac muscle.

     Hatae J, Kawata H

     Jpn J Physiol 1997 Aug 47:4 377-83.

  10. Possible mechanism of palytoxin-induced Ca++ mobilization in porcine coronary

     artery.

     Ishii K, Ito KM, Uemura D, Ito K

     J Pharmacol Exp Ther 1997 Jun 281:3 1077-84.

  11. Lipofection of a cDNA plasmid containing the dystrophin gene lowers

     intracellular free calcium and calcium leak channel activity in mdx myotubes.

     McCarter GC, Denetclaw WF Jr, Reddy P, Steinhardt RA

     Gene Ther 1997 May 4:5 483-7 .

  12. Potent vasoconstrictor actions of cyclopiazonic acid and thapsigargin on femoral

     arteries from spontaneously hypertensive rats.

     Nomura Y, Asano M, Ito K, Uyama Y, Imaizumi Y, Watanabe M

     Br J Pharmacol 1997 Jan 120:1 65-73.

 13. Increased Ca2+ influx in the resting state maintains the myogenic tone and

     activates charybdotoxin-sensitive K+ channels in femoral arteries from young

     SHR.

     Asano M, Nomura Y, Hayakawa M, Ito KM, Ito K, Uyama Y, Imaizumi Y, Watanabe M

     Clin Exp Pharmacol Physiol Suppl 1995 1: S225-7.

  14. Prolonged adverse effects of benzalkonium chloride and sodium dodecyl sulfate

     in a primary culture system of rabbit corneal epithelial cells.

     Grant RL, Acosta D

     Fundam Appl Toxicol 1996 Sep 33:1 71-82.

  15. Increased inhibitory effect of phorbol ester on cytosolic Ca2+ level and

     contraction in rat myometrium after gestation.

     Kim BK, Ozaki H, Hori M, Karaki H

     Jpn J Pharmacol 1996 Oct 72:2 111-8

  16. Possible mechanism of the potent vasoconstrictor actions of ryanodine on

     femoral arteries from spontaneously hypertensive rats.

     Asano M, Kuwako M, Nomura Y, Ito KM, Ito K, Uyama Y, Imaizumi Y, Watanabe M

     Br J Pharmacol 1996 Jun 118:4 1019-27

  17. A critical evaluation of resting intracellular free calcium regulation in

     dystrophic mdx muscle.

     Hopf FW, Turner PR, Denetclaw WF Jr, Reddy P, Steinhardt RA

     Am J Physiol 1996 Oct 271:4 Pt 1 C1325-39

  18. Superficial sarcoplasmic reticulum calcium buffering of resting,

     voltage-dependent Ca++ influx in rat femoral arterial smooth muscle.

     Nomura Y, Asano M, Ito K, Uyama Y, Imaizumi Y, Watanabe M

     J Pharmacol Exp Ther 1996 Nov 279:2 830-7.

  19. Selective inhibition of myosin phosphorylation and tension of hyperplastic

     arteries by the kinase inhibitor HA1077.

     Seto M, Shindo K, Ito K, Sasaki Y

     Eur J Pharmacol 1995 Mar 24 276:1-2 27-33 .

  20. Increased Ca2+ influx in the resting state maintains the myogenic tone and

     activates charybdotoxin-sensitive K+ channels in femoral arteries from young

     SHR.

     Asano M, Nomura Y, Hayakawa M, Ito KM, Ito K, Uyama Y, Imaizumi Y, Watanabe M

     Clin Exp Pharmacol Physiol 1995 Dec 22 Suppl 1: S225-7

  21. Increased function of voltage-dependent Ca++ channels and Ca(++)-activated

     K+ channels in resting state of femoral arteries from spontaneously hypertensive

     rats at prehypertensive stage. Asano M, Nomura Y, Ito K, Uyama Y, Imaizumi Y, Watanabe M

     J Pharmacol Exp Ther 1995 Nov 275:2 775-83.

22. Stimulation of proximal convoluted tubule phosphate transport by epidermal

     growth factor: signal transduction.

     Quigley R, Kennerly DA, Sheu JN, Baum M

     Am J Physiol 1995 Sep 269:3 Pt 2 F339-44.