Product Specification and Analysis

Product Specification and Analysis


PRODUCT NAME:	FURA-PE3(AM)*

	C₅₅H₆₅N₅O₂₉
	M.W. 1260 g/mol
	K_d = 250 nM (pH 7.2, 37°C)
	Store Frozen (-20°C)
	PROTECT FROM LIGHT
	Soluble in DMSO
DESCRIPTION:	Pale yellow gum. Cell permeable acetoxymethyl ester (AM) derivative of Fura-PE3 with spectral properties similar to the parent compound.
CATALOG NUMBER:	0108 & 0109
APPEARANCE:	Pale yellow gum
TLC :	2:1 Ethyl Acetate: Hexane Rf = 0.65 Purity: 100%
ABSORPTION SPECTRUM:	l max: 371 nm Extinction for AM ester : 33,000 M^-1cm^-1
	lmax (no Ca²⁺): 502 nm (after hydrolysis)
EXCITATION:	(no Ca²⁺): 380 nm (after hydrolysis) (high Ca²⁺): 340 nm (after hydrolysis)
HPLC: Solvent System: Column:	Purity: ~95% Water/MeOH gradient 40-100% C₁₈

Fura-PE3 References

1. Ristocetin-mediated interaction of human von Willebrand factor with platelet *The wavelengths after hydrolysis are

glycoprotein lb evokes a transient calcium signal: observations with Fura-PE3. The same as Fura-2

Milner EP, Zheng Q, Kermode JC

J Lab Clin Med 1998 Jan 131:1 49-62.

2. Calcium compartments in vascular smooth muscle cells as detected by aequorin

signal.

Abe F, Mitsui M, Karaki H, Endoh M

Br J Pharmacol 1995 Dec 116:7 3000-4.

3. [Effect of discodermin A, an antimicrobial peptide, on the cytoplasm membrane]

Sato K, Horibe K, Saito-Mitsui M, Hori M, Matsunaga S, Fusetani N, Ozaki H, Karaki H

Nippon Yakurigaku Zasshi 1997 Oct 110 Suppl 1: 199P-204P.

4. New fluorescent calcium indicators designed for cytosolic retention or

measuring calcium near membranes.

Vorndran C, Minta A, Poenie M

Biophys J 1995 Nov 69:5 2112-24.

5. Palytoxin-induced increase in endothelial Ca2+ concentration in the rabbit

aortic valve.

Amano K, Sato K, Hori M, Ozaki H, Karaki H

Naunyn Schmiedebergs Arch Pharmacol 1997 Jun 355:6 751-8.

6. Mechanism of contraction induced by bradykinin in the rabbit saphenous vein.

Eguchi D, Nishimura J, Kobayashi S, Komori K, Sugimachi K, Kanaide H

Br J Pharmacol 1997 Feb 120:3 371-8.

7. Calcium-independent regulation of pigment granule aggregation and dispersion

in teleost retinal pigment epithelial cells.

King-Smith C, Chen P, Garcia D, Rey H, Burnside B

J Cell Sci 1996 Jan 109 ( Pt 1): 33-43.

8. Intracellular calcium release is more efficient than calcium influx in stimulating

mitochondrial NAD(P)H formation in adrenal glomerulosa cells.

Rohács T, Tory K, Dobos A, Spaet, A

Biochem J 1997 Dec 1 328 ( Pt 2): 525-8.

9. Participation of PKC in modulation of the excitation-contraction process of

chick embryo cardiac muscle.

Hatae J, Kawata H

Jpn J Physiol 1997 Aug 47:4 377-83.

10. Possible mechanism of palytoxin-induced Ca++ mobilization in porcine coronary

artery.

Ishii K, Ito KM, Uemura D, Ito K

J Pharmacol Exp Ther 1997 Jun 281:3 1077-84.

11. Lipofection of a cDNA plasmid containing the dystrophin gene lowers

intracellular free calcium and calcium leak channel activity in mdx myotubes.

McCarter GC, Denetclaw WF Jr, Reddy P, Steinhardt RA

Gene Ther 1997 May 4:5 483-7 .

12. Potent vasoconstrictor actions of cyclopiazonic acid and thapsigargin on femoral

arteries from spontaneously hypertensive rats.

Nomura Y, Asano M, Ito K, Uyama Y, Imaizumi Y, Watanabe M

Br J Pharmacol 1997 Jan 120:1 65-73.

13. Increased Ca2+ influx in the resting state maintains the myogenic tone and

activates charybdotoxin-sensitive K+ channels in femoral arteries from young

SHR.

Asano M, Nomura Y, Hayakawa M, Ito KM, Ito K, Uyama Y, Imaizumi Y, Watanabe M

Clin Exp Pharmacol Physiol Suppl 1995 1: S225-7.

14. Prolonged adverse effects of benzalkonium chloride and sodium dodecyl sulfate

in a primary culture system of rabbit corneal epithelial cells.

Grant RL, Acosta D

Fundam Appl Toxicol 1996 Sep 33:1 71-82.

15. Increased inhibitory effect of phorbol ester on cytosolic Ca2+ level and

contraction in rat myometrium after gestation.

Kim BK, Ozaki H, Hori M, Karaki H

Jpn J Pharmacol 1996 Oct 72:2 111-8

16. Possible mechanism of the potent vasoconstrictor actions of ryanodine on

femoral arteries from spontaneously hypertensive rats.

Asano M, Kuwako M, Nomura Y, Ito KM, Ito K, Uyama Y, Imaizumi Y, Watanabe M

Br J Pharmacol 1996 Jun 118:4 1019-27

17. A critical evaluation of resting intracellular free calcium regulation in

dystrophic mdx muscle.

Hopf FW, Turner PR, Denetclaw WF Jr, Reddy P, Steinhardt RA

Am J Physiol 1996 Oct 271:4 Pt 1 C1325-39

18. Superficial sarcoplasmic reticulum calcium buffering of resting,

voltage-dependent Ca++ influx in rat femoral arterial smooth muscle.

Nomura Y, Asano M, Ito K, Uyama Y, Imaizumi Y, Watanabe M

J Pharmacol Exp Ther 1996 Nov 279:2 830-7.

19. Selective inhibition of myosin phosphorylation and tension of hyperplastic

arteries by the kinase inhibitor HA1077.

Seto M, Shindo K, Ito K, Sasaki Y

Eur J Pharmacol 1995 Mar 24 276:1-2 27-33 .

20. Increased Ca2+ influx in the resting state maintains the myogenic tone and

activates charybdotoxin-sensitive K+ channels in femoral arteries from young

SHR.

Asano M, Nomura Y, Hayakawa M, Ito KM, Ito K, Uyama Y, Imaizumi Y, Watanabe M

Clin Exp Pharmacol Physiol 1995 Dec 22 Suppl 1: S225-7

21. Increased function of voltage-dependent Ca++ channels and Ca(++)-activated

K+ channels in resting state of femoral arteries from spontaneously hypertensive

rats at prehypertensive stage. Asano M, Nomura Y, Ito K, Uyama Y, Imaizumi Y, Watanabe M

J Pharmacol Exp Ther 1995 Nov 275:2 775-83.

22. Stimulation of proximal convoluted tubule phosphate transport by epidermal

growth factor: signal transduction.

Quigley R, Kennerly DA, Sheu JN, Baum M

Am J Physiol 1995 Sep 269:3 Pt 2 F339-44.