Product Specification and
Analysis
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PRODUCT NAME: |
INDO-1 (AM) |
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LOT: |
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C47H51N3O22 |
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M.W. 1009.92 g/mol |
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Kd =
230 nM (After hydrolysis)* |
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Store Frozen
(-20°C) |
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PROTECT FROM
LIGHT |
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Soluble in DMSO |
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CAS Number/Name: |
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DESCRIPTION: |
Yellow solid. Cell-permeable acetoxymethyl ester (AM)
derivative of Indo-1 with spectral properties similar to the parent compound.
Insensitive to magnesium. |
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CATALOG
NUMBER: |
0105, 0106 |
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APPEARANCE: |
Yellow Solid |
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TLC : |
Ethyl Acetate: Hexane 3:2 Rf = Purity: > 95% |
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ABSORPTION
SPECTRUM: |
l max: 354 nm (Ethyl Acetate) Extinction: 33,000 M-1cm-1 |
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EMISSION
SPECTRUM: |
lmax: 405/485 nm (after hydrolysis)* |
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EXCITATION: |
354 nm (after hydrolysis)* |
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HPLC: Solvent
System: Column: |
Purity: >95% Water/MeOH gradient 40-100% C18 |
* Indo-1 AM does
not bind calcium. Only after hydrolysis to the Indo-1 cation is fluorescence
observed.